Differing Effects of Water Differing effects of water-soluble and fat-soluble extracts from Japanese radish (Raphanus sativus) sprouts on carbohydrate and lipid metabolism in normal and streptozotocin-induced diabetic rats. J Nutr Sci Vitaminol (Tokyo). 2007 Jun;53(3):261-6. Taniguchi H, Muroi R, Kobayashi-Hattori K, Uda Y, Oishi Y, Takita T. Department of Nutrition, Food Science and Culinary Arts, Toita Women's College, 2-21 17 Shiba, Minato-ku, Tokyo 105-0014, Japan. We have shown previously that Japanese radish (Raphanus sativus) sprouts (JRS) improve blood glucose levels in diabetic rats. In this study, we investigated the components in JRS that caused this hypoglycemic effect, by examining the effects of water-soluble (WSE) and fat-soluble (FSE) extracts of JRS on diabetes markers in normal (NM) and streptozotocin (STZ)-induced diabetic (DM) rats. The NM and DM rats were divided into a control group and 2 test groups (WSE (2.2%) or FSE (0.2%)), with the rats (n = 6/group) then being maintained for 3 wk on either a control diet or one of the test diets; this was followed by the
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Residues of Five Pesticides in Field Residues of five pesticides in field-treated alfalfa seeds and alfalfa sprouts. J Environ Sci Health B. 1985 Aug;20(4):445-56. Archer TE, Gauer WO. Residues of five different pesticides applied to alfalfa seed crops were determined in the harvested seeds and in sprouts grown from these seeds. Although seeds are usually used for future production of alfalfa plants, some of these seeds may be sprouted for human food consumption. The pesticides studied--aldicarb (Temik), chlorothalonil (Bravo), chlorpyrifos (Lorsban), methamidophos (Monitor) and propargite (Comite)--were applied at a normal usage rate and at two to three times that rate. Residues on the seeds and sprouts, if any, were insignificant at rates of application.
Lab Supplies ISS has been a pioneer in the creation of laboratory testing procedures and HACCP plans in the sprout industry. Since in-house labs can save growers thousands of dollars per year, ISS offers lab equipment and supplies in small quantities, saving small or emerging growers the expense of large initial orders. ISS pathogen tests have been selected for both accuracy and ease of use, and are backed by ISS technical support. Lab Equipment Variable Temperature Incubator The ISS Variable Temperature Incubator comes complete with (2) adjustable shelves and thermometer. Featuring steel interior walls, the unit is capable of holding up to (4) shelves. Pathogen Test Kits & Supplies E.coli 0157:H7 Test Kit The ImmunoCard STAT! E-Coli 0157:H7 test is a fast, flexible and accurate test for the detection of Escherichia coli 0157:H7 in food products. Each kit contains 30 tests, positive and negative controls and 40 transfer pipettes. Materials not included are flasks, autoclave, timer, scales, enrichment broth and an incubator. Oxoid Salmonella Rapid Test Kit Accurate and easy
Protective Cultures for Controlling of Microbiological Risks in Sprouting Protective Cultures for Controlling of Microbiological Risks in Sprouting In: Abstracts of the ASM 99th General Meeting, Chicago, Illinois. American Society for Microbiology 1999:522-523. E. SKYTTA1*, A. HAIKARA'.A. SIITONEN2,M. SAARELA1 AND T. MATTILA-SANDHOLM1 'VTT Biotech. Food Res.,Espoo,Finland2 Natl. Publ. Hlth Inst., Helsinki, Finland Contaminated alfalfa seed lots have been identified as the causative vehicle in a number of sprout-borne Salmonella and EHEC outbreaks. Several disinfection procedures have been recommended for the treatment of seeds intended for sprouting. All these procedures have negative counter effects which can drastically reduce the germination degree of the seeds. A range of LAB strain candidates applicable for protective cultures in sprouting were screened. The LAB strains were either isolated from a production scale sprouting process of Mung bean and Persian clover (Lactobacillus plantarum, Pediococcus pentosaceus, Lactococcus lactis subsp. lactis), or selected from a culture collection (Lactobacillus plantarum, Pediococcus parvulus, Lactococcus lactis). Antimicrobial activity of the cell free culture filtrates of LAB was tested by automated turbidometry using Salmonella isolates from sprout borne outbreaks (Salmonella enterica serovars Java, Bovismorbificans and
Alfalfa Sprouts Connected with Prevention of Menopausal SymptomsOsteoporosis AlfalfaSprouts Connected with Prevention of Menopausal Symptoms Osteoporosis, Cancerand Heart Disease ANews Extract from the Annual Review of Nutrition 17:353-3811997 DietaryPhytoestrogens ByKurtzer MS,Xu X Studiesin humans, animals and cell culture systems suggest that dietaryphytoestrogens play an important role in prevention of menopausal symptoms,osteoporosis, cancer and heart disease. Broadly defined;, phytoestrogens include isoflavones, coumestans, andlignans. Alfalfa sprouts,soybeans, clover and oilseeds (such as flaxseed) are the most significantdietary sources of isoflavones, coumestans, and lignans, respectively. A number of these compounds have been identified in fruits, vegetablesand whole grains commonly consumed by humans. Althoughthere currently are no dietary recommendations for individual phytoestrogens,there may be great benefit in increased consumption of plant foods; especiallysprouts such as Alfalfa, Clover and Soybean, and flaxseed. Proposed mechanismsinclude estrogenic and antiestrogenic effects, induction of cancer celldifferentiation, inhibition of tyrosine kinase and DNA topoisomerase activities,suppression of angiogenesis, and antioxidant effects.
Bean Sprout Water Recycling Recycle Your Bean Sprout Irrigation Water Water and energy conservation are major issues for every sprout growerlarge and small.Water supply, water disposal, and water heating costs are growing every year.Several countries, including parts of the U.S., have passed legislation to limitthe use of water. Savings of thousands of dollars can be realizedannually with theEncoreSystem. The Sungarden Sprout Division of ISS recycles 90% of its water. This water is applied directly to our growing cropsover and over without the need to exchange water. The system continues to recover water, and at the same time the growing cropsraise the temperature of the circulated water, which reduces the amount of energyrequired to heat the incoming water. So, the Encore saves you in twoways. It drastically reduces your water and sewer bill, and it reduces theamount of energy necessary for heating your incoming water supply. Tests have shown that the water recovered from the Sentrex Bean Sprouting System isclean and completely free from harmful bacteria and chemicals. What is moreimportant, the bean sprouts
Radiation Processing for Elimination of Salmonella Typhimurium From Inoculated Seeds Used for Sprout Making in India and Effect of Radiation processing for elimination of Salmonella typhimurium from inoculated seeds used for sprout making in India and effect of irradiation on germination of seeds Journal of Food Protection, Volume 70, Number 8, August 2007 , pp. 1961-1965(5) Saroj, Sunil D.; Hajare, Sachin; Shashidhar, R.; Dhokane, Varsha; Sharma, Arun; Bandekar, Jayant R. The effect of radiation processing on the germination of the sprout seeds mung (Phaseolus aureus), matki (Phaseolus aconitifolius), chana (Cicer arietinum), and vatana (Pisum sativum) in terms of percent germination, germination yield, sprout length, vitamin C content, and texture was investigated. Gradual decreases in the percent germination, germination yield, and sprout length with increases in radiation dose (0.5 to 2.0 kGy) were observed. Vitamin C content and texture remained unaffected for the seeds treated with doses of up to 2 kGy. To determine the efficacy of radiation treatment in elimination of foodborne pathogens, seeds inoculated with 4 log CFU/g of
Reactive Arthritis Following an Outbreak of Salmonella Bovismorbificans Infection From Sprouted Alfalfa Seeds in Norway Reactive arthritis following an outbreak of Salmonella Bovismorbificans infection. J Infect. 1998 May;36(3):289-95. Mattila L, Leirisalo-Repo M, Pelkonen P, Koskimies S, Granfors K, Siitonen A. Laboratory of Enteric Pathogens, National Public Health Institute, Helsinki, Finland. A large, single-source Salmonella outbreak caused by a rare serovar Bovismorbificans (6,8:r:1,5) occurred in southern Finland in 1994. The origin of the outbreak was sprouted alfalfa seeds. A questionnaire was mailed to all 210 subjects with positive stool culture. Ninety-one percent (191/210) returned the questionnaire. One hundred and fifty-three (80%) were adults. One hundred and fifty-nine out of one hundred and ninety-one (83%) reported diarrhoea, 109 (57%) fever, 104 (54%) abdominal pains, 83 (43%) fatigue, 66 (35%) articular symptoms and 20 (10%) had no symptoms. The median duration of diarrhoea was 5 days (range 1-35), that of other symptoms 4 days (range 1-30). Those reporting articular symptoms were examined (51 patients) or contacted by telephone (13 patients). Twelve percent (22/191)
Inactivation of Ecoli and Salmonella On Mung Beans Alfalfa and Other Seed Types Destined for Sprout Production Inactivation of Escherichia coli O157:H7 and Salmonella on mung beans, alfalfa, and other seed types destined for sprout production by using an oxychloro-based sanitizer Journal of Food Protection Volume 69, Number 7 pp. 1571-1578(8) 01.jul.06 Kumar, M.; Hora, R.; Kostrzynska, M.; Waites, W.M.; Warriner, K. The efficacy of a stabilized oxychloro-based food grade sanitizer to decontaminate seeds destined for sprout production has been evaluated. By using mung bean seeds as a model system, it was demonstrated that the sanitizer could be used to inactivate a five-strain cocktail of Escherichia coli O157:H7 or Salmonella introduced onto beans at 103 to 104 CFU/g. Salmonella was more tolerant to stabilized oxychloro than was E. coli O157:H7, with sanitizer levels of 150 and 50 ppm, respectively, being required to ensure pathogen-free sprouts. The decontamination efficacy was also found to be dependent on treatment time ( 8 h optimal) and the seed-to-sanitizer ratio ( 1:4 optimal). Stabilized oxychloro
Salmonella Infections Associated with Mung Bean Sprouts Salmonella infections associated with mung bean sprouts: epidemiological and environmental investigations. Epidemiol Infect. 2008 Feb 25;:1-10 Mohle-Boetani JC, Farrar J, Bradley P, Barak JD, Miller M, Mandrell R, Mead P, Keene WE, Cummings K, Abbott S, Werner SB; for the Investigation Team. California Department of Public Health, Richmond, CA, USA. SUMMARY We investigated an outbreak of Salmonella Enteritidis (SE) infections linked to raw mung bean sprouts in 2000 with two case-control studies and reviewed six similar outbreaks that occurred in 2000-2002. All outbreaks were due to unusual phage types (PT) of SE and occurred in the United States (PT 33, 1, and 913), Canada (PT 11b and 913), and The Netherlands (PT 4b). PT 33 was in the spent irrigation water and a drain from one sprout grower. None of the growers disinfected seeds at recommended concentrations. Only two growers tested spent irrigation water; neither discarded the implicated seed lots after receiving a report of Salmonella contamination. We found no difference in