Guilty by Association Guilty byAssociation SproutNet International Specialty Supply February 19, 2002 Dear Bob "Whenthere is a sprout outbreak all growers who have the same lot number are involvedin the recall of seed and sprouts.  We handle ALL our seed as though it iscontaminated.  We sanitize it using the method recommended by the FDA andwe post test it using the FDA recommended method.  We have never had an outbreakyet I am concerned that we could be guilty by association.  Is thereanything we can do to disassociate ourselves from an outbreak if a grower withthe same seed lot is not as conscientious as we are?" Dear Grower A keyprinciple of HACCP is to insure that the ingredients that go into thefinished product are of the highest quality, and to have documentation on filethat verifies the food safety and quality requirements. Butthere is more to it than just seed sanitizing and post testing.  Purchasing seedcan be likened to flying on a plane.  You need to feel confident thepassengers you share the plane with are safe and the pilot knows howto fly.  If the plane

Mechanism for the Increased Defecation and Jejunum Mucosal Protein Content in Rats by Feeding Germinated Barley Foodstuff Mechanism for the increased defecation and jejunum mucosal protein content in rats by feeding germinated barley foodstuff. Biosci Biotechnol Biochem. 1997 Mar;61(3):443-8. Kanauchi O, Agata K, Fushiki T. Applied Bioresearch Center, Kirin Brewery Co., Ltd., Gunma, Japan. We investigated the effects of germinated barley foodstuff (GBF) on the fecal excretion and jejunum mucosal protein content in male Sprague-Dawley rats fed on various diets with the same protein and dietary fiber levels. Under these experimental conditions, GBF was confirmed to induce greater fecal output compared with commercial water-soluble or -insoluble dietary fibers. While the dietary fiber extracted from GBF increased the fecal output and mucosal protein content, the protein fraction of GBF degraded to the peptide form did not increase the fecal output or mucosal protein content. Increased mucosal protein and fecal output were thus found to require the presence of the dietary fiber fraction or possibly the protein fraction bound tightly to the

Isoenzyme Composition and Some Properties of Pea Lipoxygenase Biokhimiia 1977 Nov Isoenzyme composition and some properties of pea lipoxygenase Biokhimiia 1977 Nov;42(11):2079-85 [Article in Russian] Borisova IG, Chepurenko NV, Budnitskaia EV. The isoenzyme composition and some properties of lipoxygenase isolated from the seeds and 10-day old sprouts of pea plant were studied. The enzyme activity assay, using gel- and ion-exchange chromatography, disc-electrophoresis in polyacrylamide gel, etc. revealed that the plant contains two lipoxygenase systems of unsaturated long-chain fatty acids oxidation. The existence of four lipoxygenase isoenzymes whose combination determines the type of lipoxygenase-catalyzed reactions of linoleic acid oxidation, has been confirmed.

Sulforaphane Induces G2 Sulforaphane induces G2-M arrest and apoptosis in high metastasis cell line of salivary gland adenoid cystic carcinoma. Oral Oncol. 2009 Nov;45(11):998-1004. Epub 2009 Jul 8. Chu WF, Wu DM, Liu W, Wu LJ, Li DZ, Xu DY, Wang XF. Department of Pharmacology, Harbin Medical University, Baojian Road 157, Harbin, Heilongjiang 150081, PR China. New chemotherapeutic strategy should be investigated to enhance clinical management in salivary gland adenoid cystic carcinoma (ACC). Recently, sulforaphane (SFN), as a natural compound from cruciferous vegetables exhibits a potent anti-cancer activity in various tumor cells, but remains uncertain in ACC cells. The present study examined whether SFN suppresses proliferation and in ACC cells, if so, the possible molecular targets would be further investigated. Cell survives, apoptosis, cell cycle progression and molecular targets were identified by multiple detecting techniques, including trypan blue dye exclusion assay, electron microscopy, AO/EB staining, flow cytometry and immunoblotting in human lung high metastasis cell line of salivary gland adenoid cystic carcinoma (ACC-M). The results showed that 5-20 microM SFN

Germinated Barley Foodstuffs Attenuate Colonic Mucosal Damage and Mucosal Nuclear Factor Kappa B Activity in a Spontaneous Colitis Germinated barley foodstuffs attenuate colonic mucosal damage and mucosal nuclear factor kappa B activity in a spontaneous colitis model J Gastroenterol Hepatol. 1999 Dec;14(12):1173-9 Kanauchi O, Andoh A, Iwanaga T, Fujiyama Y, Mitsuyama K, Toyonaga A, Bamba T. Applied Bioresearch Center, Corporate Research and Development Division, Kirin Brewery Co. Ltd, Gunma, Japan.   BACKGROUND: Germinated barley foodstuffs (GBF), which are derived from brewer's spent grain and are a highly safe food substance, increased butyrate production in the lower intestine and prevented mucosal damage and bloody diarrhoea in an acute experimental colitis model. As human histocompatibility leucocyte antigen (HLA)-B27 transgenic rats develop spontaneous and chronic intestinal inflammation resembling ulcerative colitis, we investigated the mechanisms underlying the preventive effects of GBF against a spontaneous and chronic colitis model. Specifically, the production of bacterial butyrate and the regulation of proinflammatory cytokine production were examined.   METHODS: A GBF diet and a cellulose (CE) diet were

Germinated Barley Foodstuffs Attenuate Colonic Mucosal Damage and Mucosal Nuclear Factor Kappa B Activity in a Spontaneous Colitis Germinated barley foodstuffs attenuate colonic mucosal damage and mucosal nuclear factor kappa B activity in a spontaneous colitis model J Gastroenterol Hepatol. 1999 Dec;14(12):1173-9 Kanauchi O, Andoh A, Iwanaga T, Fujiyama Y, Mitsuyama K, Toyonaga A, Bamba T. Applied Bioresearch Center, Corporate Research and Development Division, Kirin Brewery Co. Ltd, Gunma, Japan.   BACKGROUND: Germinated barley foodstuffs (GBF), which are derived from brewer's spent grain and are a highly safe food substance, increased butyrate production in the lower intestine and prevented mucosal damage and bloody diarrhoea in an acute experimental colitis model. As human histocompatibility leucocyte antigen (HLA)-B27 transgenic rats develop spontaneous and chronic intestinal inflammation resembling ulcerative colitis, we investigated the mechanisms underlying the preventive effects of GBF against a spontaneous and chronic colitis model. Specifically, the production of bacterial butyrate and the regulation of proinflammatory cytokine production were examined.   METHODS: A GBF diet and a cellulose (CE) diet were

Impact of Nrf2 On UVB Impact of Nrf2 on UVB-induced skin inflammation/photoprotection and photoprotective effect of sulforaphane. Mol Carcinog. 2011 Jun;50(6):479-86. doi: 10.1002/mc.20725. Epub 2010 Dec 28. Saw CL,Huang MT,Liu Y, Khor TO, Conney AH, Kong AN. Center for Cancer Prevention Research, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA. Abstract Ultraviolet (UV) of sunlight is a complete carcinogen that can burn skin, enhance inflammation, and drive skin carcinogenesis. Previously, we have shown that sulforaphane (SFN) inhibited chemically induced skin carcinogenesis via nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and others have shown that broccoli sprout extracts containing high SFN protected against UV-induced skin carcinogenesis in SKH-1 hairless mice. A recent study showed that there was no difference between Nrf2 knockout (Nrf2 KO) and Nrf2 wild-type (WT) BALB/C mice after exposing to high dose of UVB. Since Nrf2 plays critical roles in the anti-oxidative stress/anti-inflammatory responses, it is relevant to assess the role of Nrf2 for photoprotection against UV. In this context, the role of Nrf2 in UVB-induced

Seed Sampling and Testing Sprout Safety Research Over the many years we have been in business, our team has collected important seed sampling research and related sprout safety research into this helpful articles database. "California Department of Public Health Advisory to Commercial Sprout Producers", May 1, 2008 "Sprouting Seed Screening Documentation", International Specialty Supply, Cookeville, TN "Comment on Produce Safety from Production to Consumption", August 2, 2004, Food and Drug Administration Docket No. 2004M-0258. International Specialty Supply. "CSPI Backs ISS Seed Screening Program as a Risk Reduction Measure for Sprouts".  Center for Science in the Public Interest, Letter to FDA, July 18, 2005. "Detection and Isolation of Salmonella from Naturally Contaminated Alfalfa Seeds Following an Outbreak Investigation", J Food Prot 1999 Jun;62(6):662-4, Inami GB, Moler SE., California Department of Health Services, Microbial Diseases Laboratory, Berkeley 94704, USA. "Evaluating Seed Borne Pathogens as a Risk Factor in Produce Outbreaks", Plant Disease Seminar, Nov, 13, 2007, Trevor Suslow, Plant Sciences and Postharvest Technologist, UC Davis. "Factors Influencing the Growth of Salmonella during