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Inactivation of Escherichia Coli O157 On Inoculated Alfalfa Seeds with Ozonated Water Under Pressure

Inactivation of Escherichia Coli O157 On Inoculated Alfalfa Seeds with Ozonated Water Under Pressure Inactivation of Escherichia Coli O157:H7 on Inoculated Alfalfa Seeds with Ozonated Water Under Pressure1 Journal of Food Safety, Vol. 22 No.2, July 2002 RATNA R. SHARMA2 and ALI DEMIRCI2,3,4 2Department of Agricultural and Biological Engineering 3Life Sciences Consortium The Pennsylvania State University LARRY R. BEUCHAT Center for Food Safety and Department of Food Science and Technology University of Georgia AND WILLIAM F. FETT United States Department of Agriculture Agricultural Research Service Eastern Regional Research Center Food Safety Intervention Technologies Research Unit Received for Publication December 21, 2001 Accepted for Publication March 27, 2002 ABSTRACT Alfalfa seeds inoculated with Escherichia coli O157:H7 (~105 CFU/g) were subjected to low hydrostatic pressure. Seeds immersed in ozonated water at 4C were held at 8 and 12-psi ozone pressure for 2, 4, 8, 16, 32, and 64 min. Alternatively, seeds were continuously sparged with ozone for up to 64 min and then held at 12 psi for 5 min. Controls consisted of sparging

Inactivation of Escherichia Coli O157 On Inoculated Alfalfa Seeds with Ozonated Water Under Pressure2018-11-10T03:50:50+00:00

GOV’T TO APPEAL RULING OVER DAMAGES FOR SPROUT GROWERS

GOV GOV'T TO APPEAL RULING OVER DAMAGES FOR SPROUT GROWERS May 29, 2003 AP TOKYO -- Ministry sources were cited as saying Thursday that the Japanese health ministry is planning to appeal a recent ruling by the Tokyo High Court ordering the state to pay damages to growers of white radish sprouts for branding the vegetable as a probable cause of a fatal outbreak of food poisoning in 1996 in Osaka. The story says that the high court on May 21 ruled that the government must pay about 16.9 million yen in compensation to growers of "Kaiware" radish sprouts for damaging their businesses by releasing unconfirmed information when it said the sprouts were the likely source of the outbreak of food poisoning in Osaka caused by the O-157 strain of E. coli bacteria. The growers had demanded that the state pay 110 million yen to make up for the lost revenue they suffered following the announcement, while the ministry had said the announcement was appropriate.  The story adds that the

GOV’T TO APPEAL RULING OVER DAMAGES FOR SPROUT GROWERS2018-11-10T03:50:50+00:00

Two Processing Methods for the Isolation

Two Processing Methods for the Isolation of Salmonella From Naturally Contaminated Alfalfa Seeds Thefollowing research was conducted in order for researchers to be ableto determine contamination levels in seed. Though it was not the intention of the research, the data also shows thatpathogens can be detected in seed with very high probabilities if enough samplesare tested.  The highlights and underlines are mine in order to make apoint at the end of the article. Research Note: TwoProcessing Methods for the Isolation of Salmonella from Naturally ContaminatedAlfalfa Seeds Journalof Food Protection: Vol. 64, No. 8, pp. 1240-1243. Gregory B. Inami, Sue Ming C. Lee, Robin W. Hogue, and Rita A. Brenden California Department of Health Services, Microbial Diseases Laboratory, 2151Berkeley Way, Berkeley, California 94704, USA Abstract-Twoprocessing methods were examined for the recovery of Salmonella fromnaturally contaminated alfalfa seed. Seed samples, from each of threeinvestigations, were processed by sprouting and shredding before preenrichmentand culture. In lot A, Salmonella serotype Newport wasisolated from 3 of 30 sample units with the sprouting method and 2 of30 with the shredding method. In lot B, three serotypes invarious combinations were

Two Processing Methods for the Isolation2018-11-10T03:50:50+00:00

Sensitive and Rapid Detection of Vero Toxin

Sensitive and Rapid Detection of Vero Toxin Sensitive and rapid detection of Vero toxin-producing Escherichia coli using loop-mediated isothermal amplification. J Med Microbiol.2007 Mar;56(Pt 3):398-406. Hara-Kudo Y, Nemoto J, Ohtsuka K, Segawa Y, Takatori K, Kojima T, Ikedo M. Division of Microbiology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. A loop-mediated isothermal amplification (LAMP) assay was developed to detect Vero toxin (VT)-producing Escherichia coli rapidly (within 60 min). The 24 strains of VT-producing E. coli were successfully amplified, but 6 strains of non-VT-producing E. coli and 46 bacterial species other than E. coli were not. The sensitivity of the LAMP assay was found to be >0.7 c.f.u. per test using serogroups O157, O26 and O111 of VT-producing E. coli; this sensitivity is greater than that obtained by PCR assay. Furthermore, the LAMP assay was examined for its ability to detect VT-producing E. coli in food because of the difficulty of detection in food samples. The recovery of VT-producing E. coli by LAMP assay from beef and

Sensitive and Rapid Detection of Vero Toxin2018-11-10T03:50:50+00:00

Foodstuff Improves Constipation Induced by Loperamide in Rats

Germinated Barley Foodstuff Improves Constipation Induced by Loperamide in Rats Germinated barley foodstuff improves constipation induced by loperamide in rats. Biosci Biotechnol Biochem. 1998 Sep;62(9):1788-90. Kanauchi O, Hitomi Y, Agata K, Nakamura T, Fushiki T. Applied Bioresearch Center, Corporate Research and Development Division Kirin Brewery Co. Ltd., Gunma, Japan. The effects of germinated barley foodstuff (GBF) derived from the aleurone and scutellum fractions of germinated barley low-lignified hemicellulose were examined in Sprague-Dawley rats with constipation induced by loperamide by addition to the diet (2 mg/kg body weight). Bowel movements were higher in the GBF-fed rats than in the cellulose-fed rats used as a control. Fecal water content was also higher in the GBF-fed rats. The concentration of short chain fatty acids in cecal content, especially butyrate, was significantly higher in the GBF-fed rats than in the cellulose-fed rats. These findings suggested that GBF helps normalize defecation not only in diarrhea but also constipation.

Foodstuff Improves Constipation Induced by Loperamide in Rats2018-11-10T03:50:50+00:00

Comparative Study On Separation and Purification

Comparative Study On Separation and Purification of Isoflavones From the Seeds and Sprouts of Chickpea by HSCCC Comparative Study on Separation and Purification of Isoflavones from the Seeds and Sprouts of Chickpea by HSCCC. Lv Q, Yang Y, Zhao Y, Gu D, He D, Yili A, Ma Q, Cheng Z, Gao Y, Aisa HA, Ito Y. J Liq Chromatogr Relat Technol. 2009 Jan 1;32(19):2879-2892. Xinjiang Key Laboratory of Plant Resources and Natural Products Chemistry, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, P.R. China. Chickpea is known as a plant that is rich in protein, carbohydrates, and nutrition, and its seeds and sprouts have been processed into various health foods. In the present study, four isoflavones were purified from the seeds and sprouts of chickpea by high speed countercurrent chromatography (HSCCC) using two biphasic solvent systems composed of n-hexane-ethyl acetate-methanol-water (5:5:5:5, v/v) and ethyl acetate-water (1:1 v/v). The results indicated that 14.2 mg of formononetin, 15.7 mg of biochanin A, 9.1 mg of ononin, 11.3 mg

Comparative Study On Separation and Purification2018-11-09T23:22:04+00:00

Sulforaphane Reduces Infarct Volume Following Focal Cerebral Ischemia in Rodents

Sulforaphane Reduces Infarct Volume Following Focal Cerebral Ischemia in Rodents Sulforaphane reduces infarct volume following focal cerebral ischemia in rodents. Neurosci Lett. 2005 Oct 15 Zhao J, Kobori N, Aronowski J, Dash PK. Vivian L. Smith Center for Neurologic Research, Department of Neurobiology and Anatomy, University of Texas Medical School, P.O. Box 20708, Houston, TX 77225, USA. Stroke is the third leading cause of death and disability in the United States. As several biochemical mechanisms have been proposed to contribute to stroke pathophysiology, treatments acting on multiple targets may be desirable. Sulforaphane (SUL), a naturally occurring isothiocyanate present in cruciferous vegetables, has been shown to induce the expression of multiple NF-E2-related factor-2 (Nrf2) responsive genes. In the present study, we demonstrate that systemically administered SUL can enter the brain as determined by increased mRNA and protein levels of the Nrf2-responsive gene heme oxygenase 1 (HO-1). Delayed administration (15min) of a single dose of SUL significantly decreased cerebral infarct volume following focal ischemia, suggesting a potential therapeutic value for this compound.   Note

Sulforaphane Reduces Infarct Volume Following Focal Cerebral Ischemia in Rodents2018-11-09T23:21:34+00:00

Should Sprouts Be Trashed

Should Sprouts Be Trashed ShouldSprouts Be Trashed? SproutNet International Specialty Supply June 5, 2002 DearBob, "Iherd that the CDC and California Department of Health are coming out withanother article trashing sprouts.  Whydo they continue to do this?" DearGrower, Healthofficials concern for consumers who eat sprouts is not ill conceived. Sprouts, grown without controls, are a serious health risk, particularlyto those with weak immune systems. Althoughthere had been a couple other outbreaks associated with sprouts throughout theworld, sprouts really did not come onto the CDC's radar until 1994 whenoutbreaks involving 282 cases of salmonella poisoning were reported in Swedenand 210 cases in Finland. Both outbreaks were associated with the consumption ofalfalfa sprouts made from seeds imported from Australia. Alfalfa sprouts were again implicated in an outbreak in Denmark in 1995. In1995, an outbreak, involving alfalfa sprouts was reported in Oregon and BritishColumbia. Another large international outbreak occurred in 1995 in Finland andthe USA (Arizona, Michigan and 15 other states) caused by alfalfa sproutscontaminated with Salmonella stanley. Atotal of 242 cases were identified in

Should Sprouts Be Trashed2018-11-10T03:50:50+00:00

Scanning Electron Microscopy of Native Biofilms On Mung Bean Sprouts

Scanning Electron Microscopy of Native Biofilms On Mung Bean Sprouts Scanning electron microscopy of native biofilms on mung bean sprouts. Can J Microbiol. 2003 Jan;49(1):45-50. Fett WF, Cooke PH. Food Safety Intervention Technologies Research Unit, Eastern Regional Research Center, Agricultural Research Service, USDA, Wyndmoor, PA 19038, USA. wfett@arserrc.gov Native biofilms present on the adaxial surface of cotyledons of mung bean sprouts (Vigna radiata) were studied by use of scanning electron microscopy. Biofilms were abundant on the cotyledon surfaces and were comprised of rod-shaped bacteria, cocci-shaped bacteria, or yeasts, often with one type of microbe predominant. In contrast to our earlier study of biofilms on green sprouts (alfalfa, clover, broccoli, and sunflower), yeast and cocci were abundant on mung bean. Filamentous fungi were not observed. Sheet-like or fibrillar material (presumably composed of secreted microbial polysaccharides, proteins, lipids, and nucleic acids) fully or partially covered the biofilms. Biofilms up to 5 mm in length were observed, and some biofilms were comprised of more than just a monolayer of microbial cells. Native biofilms

Scanning Electron Microscopy of Native Biofilms On Mung Bean Sprouts2018-11-10T03:50:50+00:00

Ammonia As a Decontaminant for Sprouted Seed

Ammonia As a Decontaminant for Sprouted Seed ResearchNote: Reduction of Escherichia coli O157:H7 and Salmonella Typhimurium inArtificially Contaminated Alfalfa Seeds and Mung Beans by Fumigation withAmmonia Journalof Food Protection: Vol. 64, No. 11, pp. 1817-1819. SakchaiHimathongkham CaliforniaDepartment of Health Services, 601 North 7th Street, Sacramento, California94234-7320 SuphachaiNuanualsuwan, Hans Riemann, and Dean O. Cliver Departmentof Population Health and Reproduction, School of Veterinary Medicine, Universityof California, Davis, California 95616, USA Abstract-Sproutseaten raw are increasingly perceived as hazardous foods because they have beenvehicles in outbreaks of foodborne disease, often involving Escherichia coliO157:H7 and Salmonella Typhimurium. Although the source of these pathogens hasnot been established, it is known that the seeds usually are alreadycontaminated at the time sprouting begins. Earlier studies had shown thatammonia was lethal to these same pathogens in manure, so it seemed reasonable todetermine whether ammonia was effective against them when associated with seedsto be used for sprouting. Experimentally contaminated (108 to 109CFU/g) and dried seeds, intended for sprouting, were sealed in glass jars inwhich 180 or 300 mg of ammonia/liter of

Ammonia As a Decontaminant for Sprouted Seed2018-11-09T23:19:37+00:00
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