Optimization of Bioactive Compounds in Buckwheat Sprouts and Their Effect On Blood Cholesterol in Hamsters Optimization of Bioactive Compounds in Buckwheat Sprouts and Their Effect on Blood Cholesterol in Hamsters. J Agric Food Chem. 2008 Jan 24 Lin LY, Peng CC, Yang YL, Peng RY. Nutrient levels in buckwheats that were maximized in day 8 sprouts (D8SP) included total phenolics, quercetin, and l-ascorbic acid, whereas those of oxalic, malic, tartaric, and citric acids, rutin, and gamma-aminobutyric acid (GABA) were found to reach maximum levels on day 10. Ethanolic extract of D8SP (2.5 mg/mL) revealed potent free-radical scavenging (FRS) and antioxidative (ANO) capabilities. However, its Fe (2+)-chelating capability was only moderate. To further study the hypolipidemic activity of D8SP, 36 Syrian hamsters were grouped into six groups and fed for 28 days, respectively, with (i) control meal, (ii) high fat plus high cholesterol meal, (iii) high fat plus high cholesterol plus 2.5% of buckwheat seeds, (iv) high fat plus high cholesterol plus 25% of buckwheat seeds, (v) high fat plus high
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Multinational Outbreak of Salmonella Enterica Serotype Newport Infections Due to Contaminated Alfalfa Sprouts Multinational Outbreak of Salmonella enterica Serotype Newport Infections Due to Contaminated Alfalfa Sprouts JAMA 1999 Jan 13;281(2):158-62 Chris A. Van Beneden, MD, MPH; William E. Keene, PhD, MPH; Robert A. Strang, MD, MHSc, FRCPC; Denise H. Werker, MD, MHSc, FRCPC; Arlene S. King, MD, MHSc, FRCPC; Barbara Mahon, MD, MPH; Katrina Hedberg, MD, MPH; Alison Bell, MD, MHSc, FRCPC; Michael T. Kelly, MD, PhD, FRCPC; Vijay K. Balan, MS; William R. Mac Kenzie, MD; David Fleming, MD CONTEXT: In December 1995, reported Salmonella enterica serotype Newport (SN) infections increased sharply in Oregon and British Columbia but not elsewhere in North America. Similar unexplained increases had been noted in 6 other states in the fall of 1995. OBJECTIVE: To determine the source of the outbreak(s). DESIGN: Case-control studies, environmental investigations, bacterial subtyping, and surveillance information review. SETTINGS: Oregon and British Columbia communities (winter 1995-1996) and Georgia, Oklahoma, Pennsylvania, Vermont, Virginia, and West Virginia (fall 1995). PARTICIPANTS: Oregon and
Survey of Retail Alfalfa Sprouts and Mushrooms for the Presence of Escherichia Coli O157 Survey of Retail Alfalfa Sprouts and Mushrooms for the Presence of Escherichia coli O157:H7, Salmonella, and Listeria with BAX, and Evaluation of this Polymerase Chain Reaction-Based System with Experimentally Contaminated Samples February 2003 Journal of Food Protection Volume: 66 Number: 2 Page: 182 -- 187 Christine M. Strapp ; Adrienne E. H. Shearer ; Rolf D. Joerger Abstract: BAX, a polymerase chain reaction (PCR)-based pathogen detection system, was used to survey retail sprouts and mushrooms for contamination with Escherichia coli O157:H7, Salmonella, Listeria spp., and Listeria monocytogenes. No Salmonella or E. coli O157:H7 was detected in the 202 mushroom and 206 alfalfa sprout samples screened. L. monocytogenes was detected in one sprout sample, and seven additional sprout samples tested positive for the genus Listeria. BAX also detected Listeria species in 17 of the mushroom samples. Only 6 of 850 PCR assays (0.7%) failed to amplify control DNA, and therefore reagent failures and the inhibition of
Diseases Sprout in Alfalfa Products SproutNet International Specialty Supply May 17, 2006 Diseases Sprout in Alfalfa Products Herald Sun May 12, 2006 SALMONELLA and listeria have been found in some alfalfa products, sparking a statewide recall in dozens of supermarkets. Eight varieties of Parilla and Woolworths brand alfalfa, with due dates up to and including May 23, will be removed from shelves. But David Muir, spokesman for manufacturer Parilla Fresh, said the products had been in supermarkets for more than two days before the recall was announced. Mr Muir said the delay was caused by a six-day wait for the results of routine health and safety checks. "We have now heightened our vigilance in that area," he said. "Parilla is working with Food Safety Victoria. An immediate voluntary recall was actioned and we are working closely with authorities to investigate more fully the cause of the problem."~ Kate Jones Parilla Fresh and Woolworths Brand Sprouts have been recalled in Australia and New Zealand, due to
Microbiological Hazards Associated with Sprouted Seeds Microbiological hazards associated with sprouted seeds Food Safety and Hygiene - A bulletin for the Australian food industry March 2005 We last discussed sprouts and foodborne disease in theNovember 2000 issue of Food Safety and Hygiene. We noted then that whilethere have been no confirmed outbreaks of foodborne illness from sprouts produced in Australia, seeds grown in Australia had been associated with outbreaks in other countries in the 1990s. Australia continues to be without reported incidents but sprouts remain a high risk product based on the number and seriousness of outbreaks reported overseas. They are one of the plant products for which the NSW Food Authority is to introduce the requirement for food safety programs. The Campden and Chorleywood Food Research Association Group has recently published a Review of the microbiological risks associated with sprouts as part of its continuing review series. This comprehensive review reiterates the key challenges associated with the safe production of sprouted seeds and some of these bear repeating here. They include: Seeds have not been
Double Whammy for Ecoli and Salmonella Double Whammy for E. coli and Salmonella Food& Nutrition Research Briefs, July 1999 USDA ARS Treating alfalfa seeds and sprouts with a combination ofirradiation and chlorine effectively safeguards them against E. coliO157:H7 and Salmonella, researchers found. Not only did the dualtreatment kill both organisms, it extended the shelf life of sprouts from aboutfive days to more than a week. This is good news for sprout growers. Since 1995,raw alfalfa sprouts have been recognized as a source of foodborne illness in theUnited States, with several outbreaks of both E. coli O157:H7 and Salmonella.Since sprouts can't withstand abrasive physical washing because of theirfragility, cleaning the seed has become the primary focus. Along with irradiation, the researchers subjected alfalfa seeds tothree concentrations of calcium hypochlorite-a chlorine source. A 3-percentweight-per-volume concentration equals about 20,000 parts per million ofavailable chlorine. When the pH is neutral, the 2.5- and 3-percentconcentrations reduced E. coli O157:H7 by 99.99 percent. The pH level isimportant because when the solution is more alkaline, such as pH 10, thechlorine would change to
Differences in Attachment of Salmonella Enterica Serovars and Escherichia Coli O157 H7 to Alfalfa Sprouts Differences in Attachment of Salmonella enterica Serovars and Escherichia coli O157:H7 to Alfalfa Sprouts Applied and Environmental Microbiology, October 2002, p. 4758-4763, Vol. 68, No. 10 American Society for Microbiology. J. D. Barak,* L. C. Whitehand, and A. O. Charkowski Produce Safety and Microbiological Research, Western Regional Research Center, USDA Agricultural Research Service, Albany, California 94710 Received 15 April 2002/ Accepted 4 July 2002 Numerous Salmonella enterica and Escherichia coli O157:H7 outbreaks have been associated with contaminatedsprouts. We examined how S. enterica serovars, E. coli serotypes, and nonpathogenic bacteria isolated from alfalfa sprouts grow on and adhere to alfalfa sprouts. Growth on and adherence to sprouts were not significantly different among different serovars of S. enterica, but all S. enterica serovars grew on and adhered to alfalfasprouts significantly better than E. coli O157:H7. E. coli O157:H7 was essentially rinsed from alfalfasprouts with repeated washingsteps, while 1 to 2 log CFU of S. enterica remained attachedper sprout. S. entericaNewport adhered to 3-day-oldsprouts as well as Pantoea agglomerans and 10-fold more than Pseudomonas putida and Rahnella aquatilis, whereas the growth rates of all four strains throughout seed sprouting were similar. S. enterica Newport and plant-associated bacteria adhered 10- to 1,000-fold more than E. coli O157:H7; however, three of
Application Of High-Hydrostatic Pressure to Inactivate Escherichia Coli O157:h7 On Alfalfa Sprout Seeds - Sprout Food Safety Research Application of high-hydrostatic pressure to inactivate Escherichia coli O157:H7 on alfalfa sprout seeds. Institute of Food Technologists Annual Meeting, New Orleans, Jun 29, 2008,Hudaa S. Neetoo, University of Delaware, Newark, DE; Mu Ye, University of Delaware, Newark, DE; Haiqiang Chen, University of Delaware, Newark, DE Alfalfa sprouts are among the most widely consumed sprouts in the United States due to their nutritional and health-promoting benefits. Sprouts eaten raw are increasingly being perceived as hazardous foods as they have been implicated inEscherichia coli O157:H7 outbreaks. Seeds usually are already contaminated at the time of sprouting and although initial pathogen contamination levels are very low, the conditions used in the sprouting process are congenial for the growth of E. coliO157:H7. The objective of our study was to evaluate the potential of using high hydrostatic pressure (HHP) technology for alfalfa seed decontamination. Alfalfa seeds (2g) inoculated with a cocktail of five strains of E. coli O157:H7 to a final level of
Preventive Effects of Germinated Barley Foodstuff On Methotrexate Preventive effects of germinated barley foodstuff on methotrexate-induced enteritis in rats. Int J Mol Med. 1998 Jun;1(6):961-6. Kanauchi O, Mitsuyama K, Saiki T, Agata K, Nakamura T, Iwanaga T. Applied Bioresearch Center, Corporate Research and Development Division, Kirin Brewery Co. Ltd., Takasaki, Gunma 370-12, Japan. The preventive effects of the dietary germinated barley foodstuff (GBF), which increases the contents of protein, RNA and DNA in the intestinal mucosa of rats on the mucosal damage and diarrhea were examined in a methotrexate (MTX)-induced enteritis model in rats. Sprague-Dawley rats intraperitoneally injected with MTX (10 mg/kg body weight) were used as an enteritis model. After consumption of diets containing GBF, glutamine or a glutamine-rich stuff (gluten), mucosal damage, contents of mucosal protein, RNA and DNA, myeloperoxidase (MPO) activity, bacterial translocation and DNA synthetic activity in the small intestine were assessed. GBF more effectively prevented diarrhea and mucosal damages, and increased mucosal protein, DNA and RNA contents than glutamine or gluten. The bacterial trans-location and elevation of
Seeds of Controversy Seeds of Controversy: Battle is GrowingBetween Farmers and Company Over Patented Broccoli Sprouts by JaneDornbusch BostonHerald Wednesday, August 8, 2001 Along with crunchy granola, sprouts once stood as the culinary embodiment oflove and peace. But now, sprout seeds are sowing dissent and anger; some sayit's nothing less than war, waged on America's farmers. At theheart of the unlikely battle are broccoli sprouts. In the early '90s,researchers at Johns Hopkins University found that sulforaphane glucosinolate, anaturally occurring compound in cruciferous vegetables such as broccoli,cabbage, kale and Brussels sprouts, is a powerful antioxidant that could play akey role in preventing cancers. Further research revealed that broccoli sprouts- tiny plants only a couple of days old - contain a much higher concentration ofSGS than mature broccoli. Thefindings were duly reported in the media and quickly forgotten by a ficklepublic. But Johns Hopkins sought and received a patent for the broccoli sprouts,which are now exclusively licensed and sold by Brassica Protection Productsunder the brand name BroccoSprouts. "Theidea of preventing cancer before