Structural Heterogeneity of Phospholipase D in 10 Dicots

Structural heterogeneity of phospholipase D in 10 dicots.

Biochem Biophys Res Commun 1996 Apr 5;221(1):31-6
Dyer JH, Zheng S, Wang X.
Department of Biochemistry, Kansas State University, Manhattan, 66506, USA.

The occurrence of multiple forms of phospholipase D (EC was investigated in different tissues of castor bean (Ricinus communis) and in other plant species. Phospholipase D variants were resolved by nondenaturing and isoelectric focusing polyacrylamide gel electrophoresis and detected by immunoblotting using anti-phospholipase D antibodies and by enzyme activity assay. Three phospholipase D variants were produced differentially in the roots, endosperm, cotyledons, and hypocotyl of 5-day postgermination seedlings of castor bean. Furthermore, different patterns of phospholipase D variants were found in the different regions of hypocotyl (elongated and hook). Multiple phospholipase D forms were found in florets of cauliflower and broccoli, leaves of cabbage, celery, tomato, and potato, and alfalfa sprouts, suggesting that structural heterogeneity of phospholipase D occurs widely in plants.