Resolution of Escherichia Coli O157 That Contaminated Radish Sprouts in Two Outbreaks by Two Dimensional Gel Electrophoresis

Resolution of Escherichia coli O157:H7 that contaminated radish sprouts in two outbreaks by two-dimensional gel electrophoresis.
Curr Microbiol 2001 Nov;43(5):311-5
Yokoyama K, Iinuma Y, Kawano Y, Nakano M, Kawagishi M, Yamashino T, Hasegawa T, Ohta M.
Department of Molecular Bacteriology, Nagoya University Postgraduate School of Medicine, Aichi, Japan.

Two-dimensional gel electrophoresis (2-DE) was performed to examine exoproteins and periplasmic proteins of Shiga toxin-producing Escherichia coli (STEC) O157:H7 strains isolated from cases associated with radish sprouts in two outbreaks. We found that STEC O157:H7 released a large number of proteins into the medium during the stationary phase of growth, as observed with 2-DE. Although pulsed-field gel electrophoresis (PFGE) patterns of STECs NGY9 (RIMD0509894), a Sakai isolate; NGY33, a Gamagoori isolate; and NGY120, a Kanagawa isolate, were all the same, comparison of 2-DE patterns of exoproteins and periplasmic proteins clarified that NGY9 was distinct from NGY33, whereas NGY33 and NGY120 were of close lineage. We therefore suggest that 2-DE analysis of exoproteins and periplasmic proteins is a powerful epidemiological method with high resolution.


Note:  A related article can be found atSprouts Cleared from Deadliest Outbreak Attributed to Sprouts