Glucosinolate Research

Glucosinolates Below are research articles on glucosinolates and their relationship to seeds, sprouts, crucifer plants, and/or cancer.  This is included the SproutNet to help promote cancer research with glucosinolates, of which crucifer sprouts, such as broccoli, cauliflower, kale, etc, are the greatest natural source. Agerbirk, N., Olsen, C.E., and Sørensen, H.  Initial and Final Products, Nitriles, and Ascorbigens Produced in Myrosinase-Catalyzed Hydrolysis of Indole Glucosinolates.  J. Agric Food Chem. 1989, 46, 1563-1571. Albert-Puleo M.  Physiological effects of cabbage with reference to its potential as a dietary cancer-inhibitor and its use in ancient medicine.  J Ethnopharmacol 1983;9:261-72 Alink, G.M., et al., Effect of cooking and of vegetables and fruit on 1,2-dimethylhydrazine-induced colon carcinogenesis in rats, 1992, in the book: Food and Cancer Prevention:  Chemical and Biological Aspects by Waldron, Johnson and Fenwick (Eds) Al-Shehbaz, I.A. and Al-Shammary, K.I. Distribution and Chemotaxonomic Significance of Glucosinolates in Certain Middle-Eastern Cruciferae. Biochem. Systemat. Ecol. 1987, 15, 559-569. Alvarez, R.A. and Moore, C.B. Quantum Yield for Production of CH3NC in the Photolysis of CH3NCS. Science

Glucosinolate Research2018-10-04T14:05:13+00:00

Phytochemicals & Disease Prevention

Foods that Have Phytochemicals - Sprout Research - Disease Prevention Each Sprout May Contain as Many Phytochemicals as an Entire Plant (and there are about 4000 baby plants in a package of sprouts)   Green vegetables, high in phytochemicals, prevent certain types of cancer.  For instance, between 1958 and 1960, Sulforaphane was isolated in species of Brassica, Eruca, and Iberis.  In 1992 Sulforaphane was identified as as a strong Phase II enzyme inducer in broccoli.   In 1994 Steve Meyerowitz, in his book  Sprout It!,  points out that sulforaphane,known to prevent cancer, is inherent in broccoli, kale, turnip, garlic, onion and Chinese cabbage. Brassica vegetables are a principal source of antioxidant vitamins.  Sprouts have advantages over mature broccoli in that they contain higher concentrations of inducers, and the inducers mainly affect phase 2 enzyme systems. Information about the role of each nutrient and phytochemical is of critical importance. Why do sprouts have higher concentrations of inducers?  There is an inverse relationship between the age of a plant and the amount of glucosinolates per gram

Phytochemicals & Disease Prevention2018-10-04T14:09:00+00:00

Benefits of ISS Screened Sprouting Seed

Benefits of ISS Screened Sprouting Seed Just as great chefs know that the most important part of the meal is to start with the best ingredients, successful sprout growers understand that the quality of the sprouts they produce can never be better than the quality of the seed they start with. Reasons to Choose ISS Screened Sprouting Seed ISS Screened Sprouting Seed is the favorite of commercial sprout growers, big and small, because we understand all the positive attributes that growers look for in their sprouting seeds: Your sprouting seed absolutely must not have any human pathogens. Your sprouting seed should not have plant pathogens. Seeds for growing sprouts should be uniform in size and color. Sprout seed should have a high quick germination. Sprouting seeds should not be scarified and should have very few damaged seeds. Sprouting seeds should have a low moisture content but not so low as to damage the seed. The sprouts produced from purchased sprouting seed should germinate uniformly and show good vigor. The sprouting

Benefits of ISS Screened Sprouting Seed2018-10-04T14:08:21+00:00

Sulforaphane & Induced Cell Growth

Sulforaphane and 2-Oxohexyl Sulforaphane and 2-oxohexyl isothiocyanate induce cell growth arrest and apoptosis in L-1210 leukemia and ME-18 melanoma cells. Oncol Rep. 2003 Nov-Dec;10(6):2045-50. Misiewicz I, Skupinska K, Kasprzycka-Guttman T. Confocal Microscopy Laboratory, National Institute of Public Health, 00-725 Warsaw, Chelmska 30/34, Poland. Flow cytometry and laser-scanning confocal microscopy were used to study the effect of sulforaphane (SFN) and 2-oxohexyl isothiocyanate on the growth and viability of mouse leukemia L-1210 and human melanoma ME-18 cells during their exponential growth. Sulforaphane belongs to a group of compounds known as isothiocyanates. Isothiocyanates mainly occur in Cruciferous family. In particular, they occur in many vegetables such as broccoli and their sprouts. SFN and 2-oxohexyl isothiocyanate are potent inducers of detoxication phase 2 enzymes in mouse tissues and murine hepatoma cells in culture. Sulforaphane was shown to induce cell growth arrest in a dose dependent manner, followed by cell death. Sulforaphane induced the cell death via an apoptotic process. Two markers of apoptosis were investigated: phosphatidylserine externalization, which occurs in the early stages of apoptosis,

Sulforaphane & Induced Cell Growth2018-10-04T14:09:42+00:00


Sulforaphane, a dietary component of broccoli/broccoli sprouts, inhibits breast cancer stem cells. Clin Cancer Res. 2010 May 1;16(9):2580-90. Epub 2010 Apr 13. Li Y, Zhang T, Korkaya H, Liu S, Lee HF, Newman B, Yu Y, Clouthier SG, Schwartz SJ, Wicha MS, Sun D. Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA.   Abstract PURPOSE: The existence of cancer stem cells (CSCs) in breast cancer has profound implications for cancer prevention. In this study, we evaluated sulforaphane, a natural compound derived from broccoli/broccoli sprouts, for its efficacy to inhibit breast CSCs and its potential mechanism.   EXPERIMENTAL DESIGN: Aldefluor assay and mammosphere formation assay were used to evaluate the effect of sulforaphane on breast CSCs in vitro. A nonobese diabetic/severe combined immunodeficient xenograft model was used to determine whether sulforaphane could target breast CSCs in vivo, as assessed by Aldefluor assay, and tumor growth upon cell reimplantation in secondary mice. The potential mechanism was investigated using Western blotting analysis and beta-catenin reporter assay.


Eating Sprouts Bladder Cancer Research

Study Regarding Eating Sprouts and Bladder Cancer Sulforaphane retards the growth of UM-UC-3 xenographs, induces apoptosis, and reduces survivin in athymic mice Nutr Res. 2012 May;32(5):374-80. Epub 2012 May 3. Wang F, Shan Y. Source: Harbin Medical University, Harbin, China. Abstract Sulforaphane (SFN), an isothiocyanate that exists exclusively in cruciferous vegetables, may be the most promising preventive agent for bladder cancer (BC) to date. We previously observed that SFN dramatically inhibits human BC T24 cells in vitro. Our hypothesis is that SFN may attenuate BC growth. To test our hypothesis, we investigated the effect of SFN on human BC UM-UC-3 cell xenografts implanted into athymic mice. Sulforaphane extract was routinely prepared in our laboratory, and its content was measured with high-performance liquid chromatography. Athymic mice were injected subcutaneously with a UM-UC-3 cell suspension (2.0×10(6) cells/200 μL per mouse) and randomly divided into 2 groups. The positive control group was orally gavaged with water, and the treatment group was orally administered SFN from broccoli sprout (12 mg/kg body weight) for 5

Eating Sprouts Bladder Cancer Research2018-10-04T14:38:08+00:00

Stress Associated Hormones

Stress-associated hormone, norepinephrine, increases proliferation and IL-6 levels of human pancreatic duct epithelial cells and can be inhibited by the dietary agent, sulforaphane. Int J Oncol. 2008 Aug;33(2):415-9 Chan C, Lin HJ, Lin J. Center for Childhood Cancer, Columbus Children's Research Institute and Department of Pediatrics, College of Medicine, Columbus, OH 43205, USA. In current literature there is evidence that psychological factors can affect the incidence and progression of some cancers. Data obtained from animal models support the hypothesis that stress can be a cofactor. The underlying mechanisms for the association between psychological factors and pancreatic cancer are very poorly understood. In this study, we examined the possible growth promoting effects of the stress-associated hormone, norepinephrine, on immortalized human pancreatic duct epithelial cells. Our results suggest that norepinephrine can increase cell proliferation of human pancreatic duct epithelial cells. We also evaluated the ability of norepinephrine to induce interleukin-6 (IL-6), interleukin-10 (IL-10), and vascular endothelial growth factor (VEGF). All of which may promote oncogenesis of immortalized human pancreatic duct epithelial cells.

Stress Associated Hormones2018-10-04T14:39:56+00:00

Sprout Food Safety Videos

Sprout Food Safety Videos As a leading consultant to the sprouts industry on matters pertaining to sprout food safety best practices, ISS is pleased to recommend this food safety training video to sprout growers and sprout producers. The California Department of Public Health, Food and Drug Branch and the U.S. Food and Drug Administration Center for Food Safety and Applied Nutrition jointly developed this sprout food safety video in cooperation with the Centers for Disease Control & Prevention, university researchers, and industry representatives to assist the industry in producing a safer product. The video is available as viewable YouTube video files. We also provide a link to order the video at the bottom of this page. Food Safety Training Video December, June 2000. Module 1 Safer Processing of Sprouts: Module 1 Introduction to Food Safety Module 2 Safer Processing of Sprouts: Module 2 Requirements and Guidelines for Sprouters Part 1 Safer Processing of Sprouts: Module 2 Requirements and Guidelines for Sprouters Part 2 Module 3 Safer Processing of Sprouts: Module

Sprout Food Safety Videos2018-10-04T14:51:47+00:00

Chlorine Calculations for Sprouts

Calculations for Calcium Hypochlorite Calculations for 20,000 PPM Calcium Hypochlorite Recommended by the FDA as a Seed Sanitizer   Chlorine 2.0% (20,000 ppm) Mixed from 65% Calcium Hypochlorite Powder Gallons of Water à 1 5 15 30 50 100 Calcium Hypochlorite Volume Weight   3.9 oz 4.1 oz 2.5 cups 20.5 oz 7.4 cups 3.8 lbs 7.4 pints 7.7 lbs 1.5 gallons 12.8 lbs 3 gallons 25.6 lbs   Please see Safer Processing of Sprouts Module 3C Seed Treatment  for mixing instructions.

Chlorine Calculations for Sprouts2018-10-04T14:54:02+00:00

Alfalfa Sprouts Nutrition

Alfalfa Sprouts Scientific Name and Nutrition Information Alfalfa sprouts are a nutrient-rich food item. This nutrition information showcases its fiber, protein, minerals, vitamins and lipids content. Alfalfa Sprouts Scientific Name:     Medicago sativa Nutrient Units Value per 100 grams of edible portion Sample Count Std. Error Proximates Water g 91.14 10 1.226 Energy kcal 29 0 Energy kj 121 0 Protein g 3.99 10 0.563 Total lipid (fat) g 0.69 10 0.141 Carbohydrate, by difference g 3.78 0 Fiber, total dietary g 2.5 0 Ash g 0.40 10 0.044 Minerals Calcium, Ca mg 32 10 4.659 Iron, Fe mg 0.96 10 0.114 Magnesium, Mg mg 27 10 3.978 Phosphorus, P mg 70 10 7.914 Potassium, K mg 79 10 9.790 Sodium, Na mg 6 10 1.094 Zinc, Zn mg 0.92 10 0.273 Copper, Cu mg 0.157 10 0.017 Manganese, Mn mg 0.188 10 0.019 Selenium, Se mcg 0.6 0 Vitamins Vitamin C, total ascorbic acid mg 8.2 10 0.678 Thiamin mg 0.076 10 0.005 Riboflavin mg 0.126 10 0.017 Niacin mg 0.481

Alfalfa Sprouts Nutrition2018-10-04T14:54:25+00:00

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