Factors Affecting Production of Extracellular Carbohydrate Complexes by Escherichia Coli O157 Factors Affecting Production of Extracellular Carbohydrate Complexes by Escherichia coli O157:H7 Int J Food Microbiol. 2004 Sep 1;95(2):189-204. Ryu JH, Beuchat LR. Center for Food Safety and Department of Food Science and Technology, University of Georgia, 1109 Experiment Street, Griffin, GA 30223-1797, USA. Production of extracellular carbohydrate complexes (ECC) by foodborne pathogens on raw fruits and vegetables may result in protection against removal or inactivation by sanitizers. The influence of environmental conditions on cell growth, the total amount of ECC produced, and the amount of ECC produced on a per cell basis by Escherichia coli O157:H7 strains ATCC 43895 (wild type) and 43895-exopolysaccharides (EPS) (natural mutant, extensive EPS producer) was studied. To determine the effects of pH on the production of ECC on a per cell basis, E. coli O157:H7 was grown aerobically at 12 and 22 degrees C on tryptic soy agar (TSA) acidified to pH 7.0, 6.5, 6.0, 5.5, 5.0, 4.5, and 4.0. Lettuce,alfalfa sprout, cantaloupe, tomato,

Dover Sales Company Dover Sales Company, Inc. Recalls Crimson Clover Seeds Safety Alerts August 6, 1999    Piedmont, OK (SafetyAlerts) - The Food and Drug Administration (FDA) today reported that Dover Sales Company, Inc. is recalling Crimson Clover Seeds, in 50 pound three ply paper bags, because the product may be contaminated with Salmonella Typhimurium. These seeds were associated with an outbreak of Salmonella Typhimurium in Colorado.   This is the first public notice issued by the FDA.   CODE Lot W8-8-CC-861. MANUFACTURER Van Dyke Seed Company, Forest Grove, Oregon.   RECALLED BY Dover Sales Company, Inc., Piedmont, Oklahoma, by telephone on June 4, 1999. Firm-initiated recall ongoing.   DISTRIBUTION Arizona, Colorado, Florida, Hawaii, Illinois, Massachusetts, Maine, Pennsylvania, Texas, Utah, Washington state, Puerto Rico.   QUANTITY 401 units were distributed.   PRODUCT   "Sprouting Clover" Clover Seeds, packaged in 3 ounce resealable clear plastic bags. Product is sold in 1999-2000 Pinetree Garden Seeds catalog as sprouting seeds, page 56, item number SP9 and as part of a "Sprouting for Health

Pre Pre-soaking of seeds enhances pressure inactivation of E. coli O157:H7 and Salmonella spp. on crimson clover, red clover, radish and broccoli seeds. Int J Food Microbiol. 2009 Dec 3. Neetoo H, Chen H. Department of Animal and Food Sciences, University of Delaware, Newark, DE 19716-2150, USA. The application of high hydrostatic pressure (HHP) at a level of 600MPa at 20 degrees C to decontaminate crimson clover, red clover, radish and broccoli seeds inoculated with E. coli O157:H7 and Salmonella were evaluated. Salmonella was generally more pressure-resistant than E. coli O157:H7 on clover and radish seeds except on broccoli seeds where the trend was reversed. In addition, the application of HHP differentially affected seeds' germinability and the order of pressure tolerance of the seeds was such that red clover>crimson clover approximately broccoli>radish seeds with final germination percentages ranging from 85-100% while their untreated counterparts had final germination percentages of 99-100%. Pre-soaking the different types of seeds in water for 30, 60 or 90min at ambient temperature followed by HHP at

Method Patents for Seed Sprouts Are Invalid Method Patents for Seed Sprouts Are Invalid Due to Features Inherent in Prior Art The Bureau of National Affairs, Inc., Washington, D.C. Volume 64, No.1587, Page 400 ISSN 0148-7965 August 30, 2002 Method patents on the production and consumption of broccoli seed sprouts with previously unrecognized but naturally occurring cancer fighting substances are invalid for anticipation, the U.S. Court of Appeals for the Federal Circuit held Aug. 21 (In re Cruciferous Sprout Litigation, Fed. Cir, 02-1031, 8/21/02). Affirming a summary judgment of invalidity, the court found that the cancer-fighting properties of the sprouts are inherent characteristics that have existed as long as the sprouts themselves.  "While [the patent holder] may have recognized something quite interesting, it simply has not invented anything new," the court said. Broccoli Sprout Patents.  Plaintiffs Brassica Protection Products LLC and Johns Hopkins University (Brassica) hold three patents (5,725,895; 5,968,567; and 5,968,505) related to the production and consumption of seed sprouts to aid the body's natural cancer fighting defense mechanism.

Impact of Some Commonly Used Egyptian Diets On Plasma Lipids Profiles of Rats Impact of some commonly used Egyptian diets on plasma lipids profiles of rats. Z Ernahrungswiss 1993 Sep;32(3):229-36 Metwalli OM, al-Okbi SY, Abbas AE. Food Science and Nutrition Department, National Research Centre, Dokki, Cairo, Egypt. Seven types of diets commonly consumed in Egypt were studied in hyperlipemic rats. Each of the seven diets were fed to a group of hyperlipemic rats. The diets contain white beans and whole wheat bread (Diet 1); cabbage and peas (2); spinach and carrots (3);whole wheat bread and bean sprouts (4); whole wheat bread, white beans and peas (5); white beans and carrots (6) and the last diet contains peas and carrots (7). The experiment continued for 5 weeks, at the end of which different plasma lipids and biological parameters were compared to hyperlipemic rats fed the control diet for 5 weeks. Results showed that plasma total cholesterol of rats of all groups showed significant decrease, except in rats fed diet 6 in which no significant change

Patent Battle a Big Victory for Farmers Patent Battle a Big Victory for Farmers Capital Press September 13, 2001 By Cookson Beecher   Agriculture scored a victory in a recent legal decision that ruled in favor of a group of farmers who were being used for patent infringement for growing and selling broccoli sprouts.   At the heart of the lawsuit, which was launched several years ago against five sprout operations across the country, is this question:  Can the discovery of a beneficial property in a food product give the discoverer of that property the right to patent the product.   In part, that's what Johns Hopkins University and Brassica Protection Products LLC were claiming in their lawsuit against the sprouters.   But last month, the United States Court of Appeal in Maryland ruled 3-0 in the sprouters' favor, striking down three of the patents involved in the case and affirming the district court's earlier ruling in the sprouters' favor.   In its conclusion, the appellate court said that the

Sugarcane and Chicken and Radish Sprouts Create a Sampling of Country Food Safety Experiences Sugarcane, chicken and radish sprouts: a sampling of country food safety experiences. J Adv Nurs 2002 Jun;38(5):433-4. One of the main purposes of the FAO/WHO Global Forum of Food Safety Regulators is to promote the exchange of information on approaches and experiences in dealing with current food safety issues of importance to public health and the international food trade. Never before has there been an opportunity for food regulators from every region of the world to meet together to discuss positive and negative experiences on food safety issues that are of concern, on national regional and global levels. More than 50 papers have been submitted by over 30 countries to the Global Forum. In the examples taken from country papers below, five developing and developed countries tell how they tackled specific foodborne disease problems or are improving food safety in general. China: Northern China suffered several outbreaks of foodborne disease due to deteriorated sugarcane contaminated by

Sprout Food-Borne Illness Outbreaks Until the development of ISS's trusted and proven process for screening sprouting seeds, sprout seeds were the root cause of many outbreaks of food-borne illness. At ISS, we have been involved in the sprouts industry since its inception and have led the industry in mitigating the risks of outbreaks and making sprouts a safe food to grow and eat. This page chronicles some of the challenges our industry faced in its earliest years. Data Concerning Historic Sprout Outbreaks According to the Center for Science in the Public Interest, in a paper called "Outbreak Alert! 2002, "Closing the Gaps in Our Federal Food-Safety Net", sprouts made up 7% of the outbreaks from fruit and vegetables during the period of 1990-2002 (September). Produce Outbreaks 1990-2002 Vegetables Potatoes 3% Mushrooms 4% Home-canned vegetables 5% Sprouts 7% Produce Dishes 9% Other Vegetables 14% Lettuce 11% Salad 28% Fruit Melon 2% Berries 4% Other Fruits 13% Total 100% Table 1. Sprouts were suspected in 7% of the produce related outbreaks from 1990 to

Inhibition of Salmonella Typhimurium and Listeria Monocytogenes in MungBean Sprouts by Chemical Treatment Inhibitionof Salmonella Typhimurium and Listeria Monocytogenes in Mung Bean Sprouts byChemical Treatment July2002 Journal of Food Protection Volume: 65 Number: 7 Page: p1088 -- p1092 Sun-Young Lee; Kyung-Mi Yun; J. Fellman; Dong-Hyun Kang This study was undertakento compare the efficacies of chlorous acid (268 ppm), sodium hypochlorite (200ppm), and lactic acid (2%) in eliminating total mesophilic microorganisms,Salmonella Typhimurium, and Listeria monocytogenes on commercial mung beansprouts immediately after treatment and during posttreatment refrigeratedstorage. Treatment with sodium hypochlorite for 10 min did not reduce the totalaerobic count. However, treatment with lactic acid and chlorous acid for 10 mininitially reduced the total aerobic count by 0.6 and 0.8 log CFU/g,respectively, and maintained the same level or a lower level of the totalaerobic count during the storage time. Treatment with chlorous acid reducedSalmonella Typhimurium from 5.0 log to undetectable levels (<0.48 log CFU/g),and the pathogen remained undetectable over a 9-day storage period. Treatmentwith lactic acid resulted in an initial 3-log reduction

Evaluation of ISO Enrichment Real Evaluation of ISO enrichment real-time PCR methods with internal amplification control for detection of Listeria monocytogenes and Salmonella enterica in fresh fruit and vegetables. Lett Appl Microbiol. 2009 Jul;49(1):105-11. Epub 2009 Apr 22. Badosa E, Chico N, Pla M, Parés D, Montesinos E. Institut de Tecnologia Agroalimentària - CeRTA-CIDSAV, Universitat de Girona, Girona, Spain. AIMS: To provide with a quick method for qualitative detection, in less than three days, of Salmonella enterica and Listeria monocytogenes in fresh fruit and vegetables. METHODS AND RESULTS: The method was based on coupling International Standard Organization (ISO) enrichment to a real-time PCR with internal amplification control (IAC), in a duplex format, without additional DNA purification. The performance was tested on different plant products. Both bacterial pathogens were consistently detected with a limit of detection (LOD) of 1 CFU in 25 g after enrichment, except for soybean sprouts. Levels of S. enterica, ranging from 1 to 10 CFU in 25 g after enrichment were detected with different enrichment broths. CONCLUSIONS: