Directions Where We Are Cookeville, Tennessee, is centrally located in the southeast region of the United States, and sits halfway between Nashville and Knoxville on I-40.  Nashville is the closest international airport, and generally the most preferred to fly in and out of. When We Are We're in the Central Time Zone (GMT -06:00); One hour earlier in the day than Knoxville or New York and two hours later than California. Where to Stay and Things to Do There is a great website with thing to do in the area calledCookevilleIsHappening.com.  It is definitely worth checking out before visiting the area. Favorite Restaurants Taiko Noodles & Sushi Bar    125 W Broad St Cookeville, TN 38501 (931)528-0345   Royal House Thai Cuisine 208 E 8th St # A Cookeville, TN 38501 (931) 526-2478   Map to ISS from Nashville   Directions to ISS from Nashville Airport     Do the Following Then Go 1: Start out going SOUTH on TERMINAL DR. <0.1 miles 2: Go STRAIGHT toward ARRIVING FLIGHTS / DEPARTING FLIGHTS.

Sprout growers are required to test for E. coli O157 H7 and Salmonella in Spent Sprout Irrigation Water or Sprouts Samples from every batch sprout products, according to the FSMA Produce Safety Rule.  The method of analysis was released in October 2015- “Testing Methodologies for E. coli O157:H7 and Salmonella species in Spent Sprout Irrigation Water (or Sprouts)" (Version 1). FDA has recently determined that specific methods are scientifically valid and at least equivalent to the published method in accuracy, precision, and sensitivity in detecting E. coli O157:H7 and Salmonella. The following link lists these methods: https://www.fda.gov/downloads/Food/FoodScienceResearch/LaboratoryMethods/UCM622917.pdf Please inform your testing laboratory if needed. One more piece of information to share - If you re-use containers at your sprout operation, the article entitled as “Minimizing Risk in Multiple-Use Containers” would be helpful.

Sprout Growers Fight Suit by Corporate Opportunists SproutGrowers Fight Suit By 'Corporate Opportunists' FromThe January 2001 Issue of Natural Foods Merchandiser Apair of longtime sprout-growers in Washington State are going to court againstone of the country's most prestigious universities to prove that you can'tpatent nature. Gregand Lorna Lynn, owners of Harmony Farms in Auburn, Wash., say they'll keepgrowing their broccoli sprouts despite a patent-infringement lawsuit filedagainst them in October in U.S. District Court in Seattle. Thecouple is being sued by Johns Hopkins University in Baltimore, one of theworld's preeminent cancer research centers, and Brassica Protection Products, acompany that sells BroccoSprouts and holds patent licenses related to JohnsHopkins' broccoli research. "Iwas given a choice [by Brassica]," Greg Lynn said. "Either join themas a licensed grower or be a defendant in a lawsuit." Lynn and his wifedeclined last summer to join Brassica--it was the second time they'd been askedto--and decided to keep selling on their own. InOctober, the Lynns joined four other sprout farmers nationwide in a legal battlewith Johns Hopkins and

Sulforaphane Suppresses Ultraviolet B Sulforaphane suppresses ultraviolet B-induced inflammation in HaCaT keratinocytes and HR-1 hairless mice. J Nutr Biochem. 2009 Jul 1. Shibata A, Nakagawa K, Yamanoi H, Tsuduki T, Sookwong P, Higuchi O, Kimura F, Miyazawa T. Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan. Ultraviolet B (UVB) irradiation induces skin damage and inflammation. One way to reduce the inflammation is via the use of molecules termed photochemopreventive agents. Sulforaphane (4-methylsulfinylbutyl isothiocyanate, SF), which is found in cruciferous vegetables, is known for its potent physiological properties. This study was designed to evaluate the effect of SF on skin inflammation in vitro and in vivo. In in vitro study using immortalized human keratinocytes (HaCaT), UVB caused marked inflammatory responses [i.e., decrease of HaCaT viability and increase of production of an inflammatory marker interleukin-6 (IL-6)]. SF recovered the cell proliferation and suppressed the IL-6 production. These anti-inflammatory effects of SF were explained by its ability to reduce UVB-induced inflammatory gene expressions [IL-6, IL-1beta and

Isothiocyanates Sensitize the Effect of Chemotherapeutic Drugs Via Modulation of Protein Kinase C and Telomerase in Cervical Cancer Isothiocyanates sensitize the effect of chemotherapeutic drugs via modulation of protein kinase C and telomerase in cervical cancer cells Mol Cell Biochem. 2009 Oct;330(1-2):9-22. Epub 2009 Apr 12. Mukherjee S, Dey S, Bhattacharya RK, Roy M. Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata, 700 026, India.   Abstract Isothiocyanates have potential chemopreventive and antitumor effects. In the present study, we examined the actions of PEITC and sulphoraphane in modulating the activity of protein kinase C (PKC) and telomerase in cervical cancer cell line HeLa. These tumor markers are highly activated in human cancers. These compound efficiently downregulated the antiapoptotic isoforms (PKC-alpha, -betaII, -epsilon, and -zeta) as well as telomerase, whereas the proapoptotic form (PKC-delta) was upregulated. Studies were performed to measure the degree of apoptotic cell death induced by either isothiocyanates alone, or in combination with adriamycin or etoposide. Apoptosis was evident from