Erucin, a new promising cancer chemopreventive agent from rocket salads, shows anti-proliferative activity on human lung carcinoma A549 cells. FoodChem Toxicol. 2009 Jul;47(7):1430-6.Epub 2009 Mar 27. Melchini A, Costa C, Traka M, Miceli N, Mithen R, De Pasquale R, Trovato A. Dip.Farmaco-Biologico,Facoltà di Farmacia, Università di Messina, VillaggioAnnunziata, 98168 Messina,Italy. Erucin (ER) is a dietaryisothiocyanate present in cruciferous vegetables, such as rocket salads (Erucasativa Mill.,Diplotaxis sp.), that has been recently considered a promising cancer chemopreventivephytochemical. Biological activity of ER was investigated on human lung adenocarcinoma A549 cells, analyzing its effects on molecular pathways involved in apoptosis and cell cycle arrest, such as PARP-1 cleavage, p53 and p21 protein expression. Our results show that ER affects the A549 cell proliferation, enhancing significantly p53 and p21 protein expression in a dose-dependent manner (p<0.001). PARP-1 cleavage occurs only after exposure to high concentrations of ER (50 microM), in accordance to previous studies showing similar bioactivity of other isothiocyanates (ITCs). Our study reports for the first time that the induction of p53, p21 and PARP-1

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