Phase 1 Study of Multiple Biomarkers for Metabolism and Oxidative Stress After One

Phase 1 study of multiple biomarkers for metabolism and oxidative stress after one-week intake of broccoli sprouts.

M Murashima, S Watanabe, XG Zhuo, M Uehara, and A Kurashige
Biofactors, January 1, 2004; 22(1-4): 271-5.

The Department of Applied Bioscience, Tokyo University of Agriculture, 1-1-1, Sakuragaoka Setagaya, Tokyo, 156-8502, Japan.

Little is known about the direct effect of broccolisprouts on human health. So we investigated the effect of broccoli sprouts on the induction of various biochemical oxidative stress markers. Twelve healthy subjects (6 males and 6 females) consumed fresh broccoli sprouts (100 g/day) for 1 week for a phase 1 study. Before and after the treatment, biochemical examination was conducted and natural killer cell activity, plasma amino acids, plasma PCOOH (phosphatidylcholine hydroperoxide), the serum coenzyme Q(10), urinary 8-isoprostane, and urinary 8-OHdG (8-hydroxydeoxyguanosine) were measured. With treatment, total cholesterol and LDL cholesterol decreased, and HDL cholesterol increased significantly. Plasma cystine decreased significantly. All subjects showed reduced PCOOH, 8-isoprostane and 8-OHdG, and increased CoQ(10)H(2)/CoQ(10) ratio. Only one week intake of broccolisprouts improved cholesterol metabolism and decreased oxidative stress markers.