Sprout Nutrition Research Regarding Sprouts and Glucosinolates

For your convenience, we have assembled a comprehensive array of research on sprouts and glucosinolates.

“4-Methylsulfanyl-3-butenyl isothiocyanate derived from glucoraphasatin is a potent inducer of rat hepatic phase II enzymes and a potential chemopreventive agent.” Arch Toxicol. 2012 Feb;86(2):183-94. Epub 2011 Sep 30. Abdull Razis AF, De Nicola GR, Pagnotta E, Iori R, Ioannides C. Molecular Toxicology Group, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK.

“Anti-cancer Potential of Asian Brassicas” RIRDC Project No DAQ-307A, June 2004 – June 2007 Dr Tim O’Hare Queensland Department of Primary Industries and Fisheries, Queensland Horticulture Institute, Gatton Research Station.

“Anti-cancer Potential of Asian Brassicas, Glucosinolates & Chemoprevention”   A report for the Rural Industries Research and Development Corporation, by Tim O’Hare, Lesleigh Force, Lung Wong and Donald Irving, December 2006.

“Antioxidant and Choleretic Properties of Raphanus sativus L. Sprout (Kaiware Daikon) Extract” J Agric Food Chem. 2006 Dec, 27;54(26):9773-9778, Barillari J, Cervellati R, Costa S, Guerra MC, Speroni E, Utan A, Iori R.

“Aqueous Extract from Spanish Black Radish (Raphanus sativus L. Var. niger) Induces Detoxification Enzymes in the HepG2 Human Hepatoma Cell Line.” J Agric Food Chem. 2007 Aug 8;55(16):6439-46. Epub 2007 Jul 6.  Hanlon PR, Webber DM, Barnes DM. Standard Process, Department of Research and Development, 1200 West Royal Lee Drive, Palmyra, Wisconsin 53156.

“Bioavailability and kinetics of sulforaphane in humans after consumption of cooked versus raw broccoli.” J Agric Food Chem. 2008 Nov 26;56(22):10505-9. Vermeulen M, Klöpping-Ketelaars IW, van den Berg R, Vaes WH. TNO Quality of Life, AJ Zeist, The Netherlands.

“Changes in the glucosinolate-myrosinase defense system in Brassica juncea cotyledons during seedling development.”, J Chem Ecol. 2002 Feb;28(2):243-56., Wallace SK, Eigenbrode SD. Department of Plant, Soil, and Entomological Sciences, University of Idaho, Moscow 83844-2339, USA.

“Chemoprotective glucosinolates and isothiocyanates of broccoli sprouts: metabolism and excretion in humans.”, Cancer Epidemiol Biomarkers Prev 2001 May;10(5):501-8, Shapiro TA, Fahey JW, Wade KL, Stephenson KK, Talalay P., Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

“Chinese cabbage extracts and sulforaphane can protect H2O2-induced inhibition of gap junctional intercellular communication through the inactivation of ERK1/2 and p38 MAP kinases.” J Agric Food Chem. 2005 Oct 19;53(21):8205-10. Hwang JW, Park JS, Jo EH, Kim SJ, Yoon BS, Kim SH, Lee YS, Kang KS.

“Cytotoxic and Antioxidant Activity of 4-Methylthio-3-butenyl Isothiocyanate from Raphanus sativus L. (Kaiware Daikon) Sprouts” J. Agric. Food Chem., 56 (3), 875-883, 2008. 10.1021/jf073123c. Alessio Papi, Marina Orlandi, Giovanna Bartolini, Jessica Barillari, Renato Iori, Moreno Paolini, Fiammetta Ferroni, Maria Grazia Fumo, Gian Franco Pedulli, and Luca Valgimigli.

“Daikon, a promising anti-cancer vegetable” RIRDC Project No DAQ-342A, June 2007,  Dr Tim O’Hare Queensland Department of Primary Industries and Fisheries, Queensland Horticulture Institute, Gatton Research Station

“Direct Antioxidant Activity of Purified Glucoerucin, the Dietary Secondary Metabolite Contained in Rocket (Eruca sativa Mill.) Seeds and Sprouts” J Agric Food Chem. 2005 Apr 6;53(7):2475-2482. Barillari J, Canistro D, Paolini M, Ferroni F, Pedulli GF, Iori R, Valgimigli L.

“Effect of methyl jasmonate on phenolics, isothiocyanate, and metabolic enzymes in radish sprout (Raphanus sativus L.).” J Agric Food Chem. 2006 Sep 20;54(19):7263-9. Kim HJ, Chen F, Wang X, Choi JH. Department of Food Science and Human Nutrition, Clemson University, Clemson, South Carolina 29634, USA.

Effect of storage, processing and cooking on glucosinolate content of Brassica vegetables.” Food Chem Toxicol. 2007 Feb;45(2):216-24. Epub 2006 Aug 30. Song L, Thornalley PJ. Disease Mechanisms and Therapeutic Research Group, Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom.

“Effects of Glucosinolate-Rich Broccoli Sprouts on Urinary Levels of Aflatoxin-DNA Adducts and Phenanthrene Tetraols in a Randomized Clinical Trial in He Zuo Township, Qidong, People’s Republic of China” Cancer Epidemiol Biomarkers Prev. 2005 Nov;14(11):2605-13.

“Effect of storage, processing and cooking on glucosinolate content of Brassica vegetables” Food Chem Toxicol. 2007 Feb;45(2):216-24. Epub 2006 Aug 30. Song L, Thornalley PJ. Disease Mechanisms and Therapeutic Research Group, Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom.

“Erucin, a new promising cancer chemopreventive agent from rocket salads, shows anti-proliferative activity on human lung carcinoma A549 cells.” Food Chem Toxicol.  2009 Jul;47(7):1430-6. Epub 2009 Mar 27. Melchini A, Costa C, Traka M, Miceli N, Mithen R, De Pasquale R, Trovato A. Dip. Farmaco-Biologico, Facoltà di Farmacia, Università di Messina, Villaggio Annunziata, 98168 Messina, Italy.

“Evaporative light-scattering analysis of sulforaphane in broccoli samples: Quality of broccoli products regarding sulforaphane contents.” J Agric Food Chem. 2006 Apr 5;54(7):2479-83. Nakagawa K, Umeda T, Higuchi O, Tsuzuki T, Suzuki T, Miyazawa T. Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.

“Glucoraphanin and 4-Hydroxyglucobrassicin Contents in Seeds of 59 Cultivars of Broccoli, Raab, Kohlrabi, Radish, Cauliflower, Brussels Sprouts, Kale, and Cabbage” Agric. Food Chem., 52 (4), 916 -926, 2004. 10.1021/jf0307189 S0021-8561(03)00718-0, Web Release Date: January 22, 2004. Leslie G. West,* Keith A. Meyer, Barbara A. Balch, Frank J. Rossi, Michael R. Schultz, and George W. Haas. Kraft Foods North America.

“Glucosinolate Compostition and Anti-Cancer Potential of Daikon and Radish Sprouts” ISHS Acta Horticulturae 765: XXVII International Horticultural Congress – IHC2006: International Symposium on Plants as Food and Medicine: The Utilization and Development of Horticultural Plants for Human Health. T.J. O’Hare, L.S. Wong, L.E. Force, C.B. Gurung, D.E. Irving, D.J. Williams.

“Glucosinolate profiling of seed and sprouts of B. oleracea varieties used for food” Scientia Horticulturae, v.114(4):234-242, 2007. Bellostas, N.; Kachlicki, P.; Sorensen, J.C.; Sorensen, H.

Glucosinolates in Brassica vegetables: The influence of the food supply chain on intake, bioavailability and human health”.  Mol Nutr Food Res. 2008 Nov 26, Verkerk R, Schreiner M, Krumbein A, Ciska E, Holst B, Rowland I, De Schrijver R, Hansen M, Gerhäuser C, Mithen R, Dekker M.

“Glucoraphasatin: chemistry, occurrence, and biological properties.” Phytochemistry. 2010 Jan;71(1):6-12. Epub 2009 Nov 4. Montaut S, Barillari J, Iori R, Rollin P. Department of Chemistry and Biochemistry, Laurentian University, 935 Ramsey Lake Road, Sudbury ON P3E2C6, Canada.

“Heating decreases epithiospecifier protein activity and increases sulforaphane formation in broccoli” Phytochemistry. 2004 May;65(9):1273-81. Matusheski NV, Juvik JA, Jeffery EH. Department of Food Science and Human Nutrition, University of Illinois, 499 Bevier Hall, 905 South Goodwin Avenue, Urbana, IL 61801, USA.

“Identification and quantification of glucosinolates in sprouts derived from seeds of wild Eruca sativa L. (salad rocket) and Diplotaxis tenuifolia L. (wild rocket) from diverse geographical locations” J Agric Food Chem. 2007 Jan 10;55(1):67-74. Bennett RN, Carvalho R, Mellon FA, Eagles J, Rosa EA. CECEA-Departamento de Fitotecnia e Engenharia Rural, Universidade de TrAs-os-Montes e Alto Douro (UTAD), Apartado 1013, 5001-801 Vila Real, Portugal.

“Influence of light on health-promoting phytochemicals of broccoli sprouts” Journal of the Science of Food and Agriculture, Volume 88, Issue 5, Date: 15 April 2008, Pages: 904-910 Santiago Pérez-Balibrea, Diego A Moreno, Cristina García-Viguera, Department of Food Science and Technology, CEBAS-CSIC, Campus Universitario de Espinardo, Murcia, Spain.

“Influence of temperature and ontogeny on the levels of glucosinolates in broccoli (Brassica oleracea Var. italica) sprouts and their effect on the induction of mammalian phase 2 enzymes.”, J Agric Food Chem 2002 Oct 9;50(21):6239-44, Pereira FM, Rosa E, Fahey JW, Stephenson KK, Carvalho R, Aires A.

“Isolation of 4-Methylthio-3-butenyl Glucosinolate from Raphanus sativus Sprouts (Kaiware Daikon) and Its Redox Properties.” J Agric Food Chem. 2005 Dec 28;53(26):9890-9896.

“Multi-targeted prevention of cancer by sulforaphane.” Cancer Lett. 2008 Oct 8;269(2):291-304. Epub 2008 May 27. Clarke JD, Dashwood RH, Ho E. Department of Nutrition and Exercise Sciences, 117 Milam Hall, Oregon State University, Corvallis, OR 97331, USA.

“Nutrition and cancer: a review of the evidence for an anti-cancer diet” Nutr J. 2004 Oct 20;3:19, Donaldson MS. Hallelujah Acres Foundation, 13553 Vantage Hwy, Ellensburg, WA 98926, USA.

“Phytochemical composition and biological activity of 8 varieties of radish (Raphanus sativus L.) sprouts and mature taproots.” J Food Sci. 2011 Jan-Feb;76(1):C185-92. doi: 10.1111/j.1750-3841.2010.01972.x. Hanlon PR, Barnes DM. Dept. of Research and Development, Standard Process, 1200 West Royal Lee Dr., Palmyra, WI 53156, USA.

“Potential health benefits of broccoli- a chemico-biological overview.” Mini Rev Med Chem. 2009 Jun;9(6):749-59. Vasanthi HR, Mukherjee S, Das DK. Department of Surgery, University of Connecticut School of Medicine, Farmington, CT 06030-1110, USA.

“Purification of sulforaphane from Brassica oleracea seed meal using low-pressure column chromatography” J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Oct 17; Liang H, Yuan Q, Xiao Q.

“Quantification of sulforaphane mercapturic acid pathway conjugates in human urine by high-performance liquid chromatography and isotope-dilution tandem mass spectrometry.” Chem Res Toxicol. 2008 Oct;21(10):1991-6. Epub 2008 Aug 26. Egner PA, Kensler TW, Chen JG, Gange SJ, Groopman JD, Friesen MD. Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.

“Quantitative determination of intact glucosinolates in broccoli, broccoli sprouts, Brussels sprouts, and cauliflower by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry” Anal Biochem. 2005 Aug 1;343(1):93-9. Tian Q, Rosselot RA, Schwartz SJ.

“Radish Sprouts Versus Broccoli Sprouts:  A Comparison of Anti-Cancer Potential Based on Glucosinolate Breakdown Products” ISHS Acta Horticulturae 841: II International Symposium on Human Health Effects of Fruits and Vegetables: FAVHEALTH 2007, T.J. O’Hare, D.J. Williams, B. Zhang, L.S. Wong, S. Jarrett, S. Pun, W. Jorgensen, M. Imsic.